#13 ✓resolved
john.mccallum (at plantandfood)

Newbler template bug???

Reported by john.mccallum (at plantandfood) | April 1st, 2011 @ 01:16 AM

[Cfljam@pgenome 40.assemble]$ moa new newbler -t 'assemble DH skim reads' Created a Moa newbler job
[Cfljam@pgenome 40.assemble]$ moa set input input:
$ ../00.raw/MID6.sff [Cfljam@pgenome 40.assemble]$ moa show input ../00.raw/MID6.sff
largecontig_cutoff
library_name $(shell echo basename $(CURDIR) | sed "s/[ \///\/]//g" )
mid_configuration
mids
min_identity
postcommand
precommand
title assemble DH skim reads
[Cfljam@pgenome 40.assemble]$ moa set library_name library_name:
$ DHSkim [Cfljam@pgenome 40.assemble]$ moa run Executing
Input files ../00.raw/MID6.sff
runAssembly -ace -o . -consed ../00.raw/MID6.sff
Error: Options -ace, -noace, -acedir, -consed and -consed16 are mutually exlusive.
Usage: runAssembly [-o projdir] [-nrm] [-p (sfffile | [regionlist:]analysisDir)]... (sfffile | [regionlist:]analysisDir)...
Traceback (most recent call last):
File "/opt/moa10/bin/fastaInfo", line 155, in

for name, seq in fastareader(inp):

File "/opt/moa10/bin/fastaInfo", line 87, in fastareader

F = open(f, 'r')

IOError: [Errno 2] No such file or directory: '454LargeContigs.fna'
make: *** [454LargeContigs.all.png] Error 1
Moa Error running run (<1 sec)
[Cfljam@pgenome 40.assemble]$ pwd /data/moa/onion/20.genomic.sequencing/40.assemble [Cfljam@pgenome 40.assemble]$ [ pgenome ][

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