Moamoa Moa

[Cfljam@pgenome 40.assemble]$ pwd /data/moa/onion/20.genomic.sequencing/40.assemble [Cfljam@pgenome 40.assemble]$ ls -la total 12
drwxrwxr-x 3 Cfljam users 4096 Apr 1 14:31 .
drwxrwxrwx 9 Cfljam users 4096 Apr 1 14:14 ..
drwxrwxr-x 4 Cfljam users 4096 Apr 1 14:31 .moa
-rw-rw-r-- 1 Cfljam users 0 Apr 1 14:31 singletons.lst [Cfljam@pgenome 40.assemble]$ moa show input ../00.raw/MID6.sff
largecontig_cutoff
library_name DHSkim
mid_configuration
mids
min_identity
postcommand awk '$2=="Singleton" {print $1}' DHSkim/454ReadStatus.txt > singletons.lst
precommand
title assemble DH skim reads
[Cfljam@pgenome 40.assemble]$ moa run Executing
Input files ../00.raw/MID6.sff
runAssembly -ace -o . -consed ../00.raw/MID6.sff
Error: Options -ace, -noace, -acedir, -consed and -consed16 are mutually exlusive.
Usage: runAssembly [-o projdir] [-nrm] [-p (sfffile | [regionlist:]analysisDir)]... (sfffile | [regionlist:]analysisDir)...
Traceback (most recent call last):
File "/opt/moa10/bin/fastaInfo", line 155, in

for name, seq in fastareader(inp):

File "/opt/moa10/bin/fastaInfo", line 87, in fastareader

F = open(f, 'r')

IOError: [Errno 2] No such file or directory: '454LargeContigs.fna'
make: *** [454LargeContigs.all.png] Error 1
awk: cmd. line:1: fatal: cannot open file `DHSkim/454ReadStatus.txt' for reading (No such file or directory)
Moa Error running run (<1 sec)
[Cfljam@pgenome 40.assemble]$