#24 ✓resolved
john.mccallum (at plantandfood)

Blast template//help and other ?

Reported by john.mccallum (at plantandfood) | May 11th, 2011 @ 11:16 PM | in v1.1

1) No 'db' entry in help for BLAST template?

2) Ungracious error ..
[Cfljam@pgenome 16.BLASTX_uniprotPlants]$ pwd /data/moa/onion/Repeats/16.BLASTX_uniprotPlants [Cfljam@pgenome 16.BLASTX_uniprotPlants]$ moa show db /data/moa/db/uniprot/20.blastdb/sprot_plants/sprot_plants.psq
eval 1e-10
gff_blasthit F
gff_source BLAST
input ../11.repeatmasker/JBVR_filtered_repeats.fasta
nohits 50
nothreads 2
outgff gff/.gff
output out/
.out
postcommand
precommand
program blastx
project repeats
title BLASTX filtered repeats against UNiprot
[Cfljam@pgenome 16.BLASTX_uniprotPlants]$

Warning: Encountered a run error?
Traceback (most recent call last):
File "/opt/moa10/bin/moa", line 318, in

run_1()

File "/opt/moa10/bin/moa", line 292, in run_1

run_2()

File "/opt/moa10/bin/moa", line 246, in run_2

run_3(wd)

File "/opt/moa10/bin/moa", line 201, in run_3

silent = sysConf.options.silent)

File "/opt/moa10/lib/python/moa/job.py", line 248, in execute

execNow, verbose = verbose, silent=silent)

File "/opt/moa10/lib/python/moa/backend/ruff.py", line 222, in execute

logger = ruffus.black_hole_logger,

File "build/bdist.linux-x86_64/egg/ruffus/task.py", line 2680, in pipeline_run ruffus.ruffus_exceptions.RethrownJobError:

Exceptions running jobs for

'def moa.backend.ruff.executor(...):'

Original exception:

Exception [#1](/projects/73665/tickets/1 "Ticket #1")
ruffus.ruffus_exceptions.JobSignalledBreak(


    ):
for moa.backend.ruff.executor.Job = [[[../11.repeatmasker/JBVR_filtered_repeats.fasta, sprot_plants.psq]] -> [gff/JBVR_filtered_repeats.gff, out/JBVR_filtered_repeats.out], #!/bin/bash -e
    blastall -i ../11.repeatmasker/JBVR_filtered_repeats.fasta -p blastx \
            -e 1e-10 -m 7 -a 2 -d /data/moa/db/uniprot/20.blastdb/sprot_plants/sprot_plants.psq \
            -b 50 -v  50 -o out/JBVR_filtered_repeats.out

    cat out/JBVR_filtered_repeats.out | blast2gff -b F  \
        -s BLAST -d query > gff/JBVR_filtered_repeats.gff, {'gff_source': 'BLAST', 'default_command': 'run', 'wd': '/data/moa/onion/Repeats/16.BLASTX_uniprotPlants', 'moa_filesets': ['input', 'db', 'outgff', 'output'], 'title': 'BLASTX filtered repeats against UNiprot', 'precommand': '', 'db': '/data/moa/db/uniprot/20.blastdb/sprot_plants/sprot_plants.psq', 'postcommand': '', 'project': 'repeats', 'nohits': '50', 'program': 'blastx', 'silent': None, 'nothreads': '2', 'eval': '1e-10', 'gff_blasthit': 'F', 'output': 'out/JBVR_filtered_repeats.out', 'outgff': 'gff/JBVR_filtered_repeats.gff', 'input': '../11.repeatmasker/JBVR_filtered_repeats.fasta'}]

Traceback (most recent call last):
  File "build/bdist.linux-x86_64/egg/ruffus/task.py", line 517, in run_pooled_job_without_exceptions
    return_value =  job_wrapper(param, user_defined_work_func, register_cleanup, touch_files_only)
  File "build/bdist.linux-x86_64/egg/ruffus/task.py", line 447, in job_wrapper_io_files
    ret_val = user_defined_work_func(*param)
  File "/opt/moa10/lib/python/moa/backend/ruff.py", line 357, in executor
    raise ruffus.JobSignalledBreak
JobSignalledBreak:

[Cfljam@pgenome 16.BLASTX_uniprotPlants]$ moa err [blastall] FATAL ERROR: R=3: Database /data/moa/db/uniprot/20.blastdb/sprot_plants/sprot_plants.psq was not found or does not exist [Cfljam@pgenome 16.BLASTX_uniprotPlants]$ [ pgenome ][ 0- db 1

Comments and changes to this ticket

  • MF

    MF May 11th, 2011 @ 11:44 PM

    • State changed from “new” to “resolved”

    Fixed - problem with parsing through the parameters & categories of parameters.

    The template field on category order is now deprecated

Please Sign in or create a free account to add a new ticket.

With your very own profile, you can contribute to projects, track your activity, watch tickets, receive and update tickets through your email and much more.

New-ticket Create new ticket

Create your profile

Help contribute to this project by taking a few moments to create your personal profile. Create your profile ยป

lightweight workflows for bioinformatics

Shared Ticket Bins

People watching this ticket

Pages