#40 new
john.mccallum (at plantandfood)

Bowtie template issue

Reported by john.mccallum (at plantandfood) | March 28th, 2012 @ 08:02 AM | in v1.1

This template doesnt respect fasta input.
Also the distinction between db and index base is rather confusing

...............................................

[cfljam@pgenome 07.Map_primers]$ head all_primers

10D12F CATCTCCATCGCTATCATCG
10D12R ACATTTCCAGTTGCCTCCAA

10E11F CCAAAGACTCGTCTGCGAAC
10E11R TCGCCAACTTAGACGATTCC

[cfljam@pgenome 07.Map_primers]$ head all_primers

10D12F CATCTCCATCGCTATCATCG
10D12R ACATTTCCAGTTGCCTCCAA

10E11F CCAAAGACTCGTCTGCGAAC
10E11R TCGCCAACTTAGACGATTCC

[cfljam@pgenome 07.Map_primers]$ moa show db L ../06.Target_Bowtie
ebwt_base L ../06.Target_Bowtie/NXD_target
fq_input L .
input L all_primers
input_format L fasta
jobid L Map_primers
title L map primers to targets
[cfljam@pgenome 07.Map_primers]$ moa run Moa:Warning: Removing a stale lockfile
Error: reads file does not look like a FASTQ file
Command: bowtie -S --un all_primers.unmapped.fq --un all_primers.unmapped.fq ../06.Target_Bowtie/NXD_target ./all_primers
[samopen] no @SQ lines in the header. [main_samview] random alignment retrieval only works for indexed BAM files. ï¿ï¿½BC!sr�ed�|�t�ï¿ï¿½BCMoa:Error: Caught a Ruffus error Moa:Error: Exceptions running jobs for 'def moa.backend.ruff.map.localMapExecutor(...
Moa:Error: ...5, in localMapExecutor raise ruffus.JobSignalledBreak JobSignalledBreak:
Moa:Error: While processing: ./all_primers
Moa: Error running run (<1 sec)
Moa:Error: Quitting

No comments found

Please Sign in or create a free account to add a new ticket.

With your very own profile, you can contribute to projects, track your activity, watch tickets, receive and update tickets through your email and much more.

Create new ticket

Create your profile

Help contribute to this project by taking a few moments to create your personal profile. Create your profile ยป

lightweight workflows for bioinformatics

Shared Ticket Bins

People watching this ticket

Tags

Pages